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KMID : 1005220160310010019
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Journal of Embryo Transfer
2016 Volume.31 No. 1 p.19 ~ p.25
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Effect of Type of Culture Media on B6D2F1 Mice Oogenesis
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Yoo Chang-Seok
Park Kee-Sang
Seo Byoung-Boo
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Abstract
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This study was conducted to evaluate the effects of type of culture media (BM, G2, OS, TCM, and MEM) on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri F1 mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. In vitro maturation was highest in BM followed by the order of OS, MEM, TCM and G2 (90±2.8% > 88±3.2% > 85±4.9% > 78±10.2% > 64±7.7%, respectively). To note, the G2 group was statistically different compared to other groups (p<0.05). On the other hand the fertilization rate was highest in the G2 group followed by BM, OS, TCM, and MEM (87±7.2% > 85±6.9% > 74±14.0% > 71±13.8% > 2±1.4%, respectively). The MEM group was significantly lower compared to other groups (p<0.05). The developmental rate was highest in the OS group followed by the G2 group and the BM group albeit no statistical significance was noted (73±11.6% > 71±9.2% > 66±10.4%). Of note, all cells of the TCM and MEM groups were died during embryonic development. The zona hatched rate (51±9.8% vs. 50±9.1% vs. 47±7.2% for BM, G2, and OS respectively) and attached rate (45±12.3% vs. 38±16.1% vs. 37±11.5% for BM, G2, and OS respectively) were not different amongst groups. No difference was found in total cell numbers (74±13.9 vs. 64±9.2 vs. 76±6.7 for BM, G2, and OS respectively), ICM cell numbers (20±1.9 vs. 14±1.8 vs. 15±2.1), TE cell numbers (55±12.5 vs. 49±10.7 vs. 61±5.9), % ICM (30±2.8% vs. 24±7.0% vs. 22.8±2.2%) and ICM:TE ratio (1:2±0.5 vs. 1:3.1±0.8 vs. 1:3.1±0.5) amongst groups. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.
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KEYWORD
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B6D2F1 mice , culture media , oogenesis , inner cell mass , trophectoderm
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